The LightCycler® 480 Instrument is intended for performing rapid, accurate polymerase chain reaction (PCR) in combination with real-time, online detection, enabling Absolute or Relative Quantification of a target nucleic acid, as well as post-PCR analysis of the amplified nucleic acid by Melting Curve analysis.
Five excitation and six emission filters allow to work with multiple assay formats, including SYBR Green I, hydrolysis probes, Molecular Beacons and others.
For more information, you can visit Roche website
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The Chromium iX, based on NextGEM technology, is a microfluidic instrument for isolating cells or cell nuclei from a suspension (biological sample) in order to carry out transcriptomics, proteomics and/or epigenetics analyzes by NGS at the scale of single cells or nuclei.
Possible and non-exhaustive analyzes using the instrument :
- Gene expression on single cells or nuclei (Single Cell Gene Expression) on all types of fresh cells, or on human and murine cells fixed with 4% PFA
- Characterization of accessible regions of chromatin (ATAC) at the scale of single nuclei, possible in combination with gene expression on cells or single nuclei
- Amplification of VDJ sequences of fresh human and murine T and B lymphocytes in order to carry out immune profiling of the organisms studied, at the scale of single cells
The Chromium iX can isolate cells/nuclei from 1 to 8 samples per run, and allows the simultaneous analysis of 500 to 80,000 unique cells or nuclei (up to 160,000 cells using multiplexing). The capture rate is a maximum of 65% and the multiplet rate is 0.8% for 1000 cells.
Technology principle
For more info you can visit 10X Genomics website
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The NextSeq 2000 Sequencing System offers innovative design features, advanced chemistry, and an intuitive workflow operating the widest range of applications on a benchtop sequencing system.
Main features :
Sequencing options (XLEAP-SBS new chemistry)
Flow cells can be used for single read (SE) or paired-end (PE) sequencing.
For example, P2 100bp flow cell can be used for SR 100bp or PE 2x50bp sequencing.
For more info you can visit Illumina website
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The Qubit™ 4 Fluorometer is a benchtop fluorometer that can be used for the
quantitation of DNA, RNA, microRNA, and protein, as well as for the measurement
of RNA integrity and quality. You can also use the Qubit™ 4 Fluorometer to directly measure the fluorescence of samples.
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The TapeStation 4200 allows for the automatic analysis of nucleic acids (DNA and RNA) by electrophoresis, simultaneously and automatically determining the size, quality (contaminants), and concentration of samples. Sample migration is performed in "ScreenTape," single-use cartridges that are specific to the type of sample (DNA, RNA) and the desired sensitivity level.
The process involves depositing or mixing 1 µl (or 2 µl for High Sensitivity ScreenTape) of the sample with the migration buffer in 0.2 ml tubes or skirted 96-well plates. The migration is then carried out on ready-to-use, miniaturized gels, capable of processing from 1 to 96 samples simultaneously at a constant cost and in less than 2 minutes per sample.
ARN analysis
DNA analysis
For more info you can visit Agilent website
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