The MACSQuant Tyto Cell Sorter is an instrument for fluorescence-based cell sorting using a patented microchip technology with a high-speed mechanical sort valve.
The MACSQuant Tyto allows to have 10 optical parameters, the BSC and SSC with PMT and 8 fluorescences:
- 2 PMT on the 405nm purple laser
- 450/50nm (VioBlue™,DAPI, Pacific Blue™,..)
- 525/50nm (VioGreen™, V500™,…)
- 4 PMT on the 488nm blue laser
- 525/50nm (Vio515™, FITC, GFP,…)
- 585/40nm (PE)
- 655-730nm (IP, PerCP-Vio700™, PerCP,…)
- 750nm Long Pass (PE-Vio770™, PE-Cy7)
- 2 PMT on the 640nm red laser
- 655-730nm (Vio667™, APC,…)
- 750nm LP (APC-Vio770™, APC-Cy7,…)
The MACSQuant Tyto measures cell size and cell complexity, like granularity, with backscatter and side scatter detectors. Backscatter detectors collect the light that is scattered back from a cell primarily by diffraction of the light around the cell. The high-frequency fluidic valves embedded in the closed cartridges have a microsecond range of valve opening and closing cycles. The MACSQuant Tyto can reach a sort rate up to 30,000 valve actuations per second (results depend on input sample and Poisson statistics). Cells pass through the microchip under low positive pressure and no sheath fluid for high cell viability and functionality, even with fragile cells. The cartridge has aseptic ports for sample administration and collection. Since the sorting process is not using droplets, no biohazardous aerosols are formed. This minimizes the operator risk. Samples are also protected from environmental contamination in the closed system of the MACSQuant Tyto. Disposable cartridges minimize sample-to-sample carryover and eliminate the need for sample loading and collection ports. Cells sorted with the MACSQuant Tyto are ready for use in sensitive downstream applications such as cell culture and cell expansion.
For more info you can visit Miltenyi Biotec website
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